2 edition of immunofluorescent localization of the LH cells of the human adenohypophysis. found in the catalog.
immunofluorescent localization of the LH cells of the human adenohypophysis.
Written in English
|Contributions||Toronto, Ont. University. Theses (M. Sc.)|
|LC Classifications||LE3 T525 MSC 1968 B35|
|The Physical Object|
|Number of Pages||122|
Development and Differentiation of Pituitary Cells. adenohypophysis of the human fetus as observed with immunocyto- Immunocytological localization of LH, FSH, TSH and their subunits. G-I, In contrast, results of dual-immunofluorescent labeling for VGLUT2 and LH (G), VGLUT2 and FSH (H), or VGLUT2 and TSH (I) reveal frequent cases of signal coexpression (yellow cells indicated.
Ovarian Cell Differentiation: A Cascade of Multiple Hormones, Cellular Signals, and Regulated Genes. namely, the suppression of the luteinizing hormone (LH) surge and, consequently, the absence of ovulation and luteinization. The LH surge is unique to the female and is the major target of oral contraceptive by: Dual-immunofluorescent studies of the hypophyses from male rats showed the presence of VGLUT2 in high percentages of LH ( ± %)-, FSH ( ± %)-, and TSH ( ± %)-immunoreactive cells and its much lower incidence in cells of Cited by:
Fifteenth report from the Committee of Public Accounts session 1985-86
Getting it right
Kelmscott Press and William Morris, master-craftsman
Sir Francis Seymour Haden
Königlich Sächsische Porzellan-Manufaktur zu Meissen.
Effects of pressure and deposit thickness on the stabilization rateof benthic marine sludge deposits
Employment profile of Hounslow
Management for athletic/sport administration
Post-graduate education in the 1980s [written by S. Blume and O. Amsterdamska].
Seats, New York
Mr. Sponges sporting tour
Job analysis and curriculum construction in the metal trades industry
Calendar of Close Rolls. Richard II, 1377-1399
List of paintings in the collection of the Hispanic society of America.
Cobalt catalysed oxidation reactions.
DOT guide to CAB sunset
The LH producing cell of the human adenohypophysis has been identified by the immunofluorescent technique followed by aldehyde thionin-periodic acid Schiff-orange G (AT-PAS-OG) staining. It is a basophilic subtype with sparse granularity which is both PAS and aldehyde by: 7.
J Clin Endocrinol Metab. Feb;30(2) Immunofluorescent localization of the LH cell of the human adenohypophysis. Bain J, Ezrin by: 7. Immunofluorescent localization of the LH cell of the human adenohypophysis. (PMID) Abstract Citations; Related Articles; Data; BioEntities; External Links ' ' Bain J, ' ' Ezrin C The Journal of Clinical Endocrinology and Metabolism [01 Feb30(2 Cited by: 7.
proc soc exp biol med. apr; immunofluorescent localization of lh and fsh in the human adenohypophysis. koffler d, fogel by: 9. Tougard, C., Kerdelhué, B., Tixier-Vidal, A., and Jutisz, M.,Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase-labeled antibody technique, J.
Cell by: 8. Pituitary glands from juvenile (pre-pubertal) and adult male and female rhesus monkeys were examined following immunocytochemical staining with antisera to the beta subunits of ovine luteinizing hormone (LHβ) and of human thyroid stimulating hormone (TSHβ).Cited by: This is a basophilic large cell type with very small granules of the hormone.
The granule size, measured by electron microscopy, is − nm. LH and FSH are located in the gonadotrope, also basophilic, containing granules of − nm. ACTH is located in the corticotropic cell of the pars distalis, a highly staining basophil, with granules − nm in diameter. MELLORS RC, ORTEGA LG, HOLMAN HR.
Role of gamma globulins in pathogenesis of renal lesions in systemic lupus erythematosus and chronic membranous glomerulonephritis, with an observation on the lupus erythematosus cell reaction. J Exp Med. Aug 1; (2)– [PMC free article] VAZQUEZ JJ, DIXON by: Marchand, C.R., Sharp, P.J.: Immunofluorescent localization and ultrastructural characterization of gonadotrophe cells in the adenohypophysis of the Barbary Drake (Cairina moschata L.) using antichicken LH serum.
Cell Tiss. Res.– () Google Scholar. In this analysis, the authors directly compared immunofluorescence and fluorescent-protein tagging of human proteins and studied their subcellular localization Cited by: Immunofluorescent Analysis of Mouse and Human Sperm Mouse and human sperm slides were rinsed in M phosphate‐buffered saline (PBS) and blocked with 10% goat or rabbit serum plus % Triton X‐ in PBS to reduce nonspecific immunostaining and incubated with primary antibody or normal nonimmune serum as a negative control at 4°C by: Immunofluorescence (IF) uses antibodies and fluorescent detection to study the localization, relative expression, and activation states of target proteins in fixed cells or tissues.
IF is widely used in diverse fields of study. IF can be used to answer questions such as: What cells. 1. Cell Tissue Res. Jul 19;(4) Immunofluorescent localization and ultrastructural characterization of gonadotrophe cells in the adenohypophysis of the barbary drake (Cairina moschata L.) using anti-chicken LH serum.
Marchand CR, Sharp by: 8. Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples.
This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample.
In the porcine pituitary, Mirecka and Pearse (), using the immunoflu-orescence technique, concluded that the majority of gonadotrophs contained both gonadotropins. However, Herlant and Ectors () and Herlant () described, at the electron-microscope level, two cells types as LH- and FSH-secreting cells.
Immunofluorescence or fluorescent antibody staining is an antigen-detection test that is used primarily on frozen tissue sections, cell “smears,” or cultured cells; formalin-fixed tissue samples are generally not useful with this procedure.
Antigen is detected through the binding to the sample matrix of specially modified, agent-specific antibodies. Bain J, Ezrin C. Immunofluorescent localization of the LH cell of the human adenohypophysis. J Clin Endocrinol Metab. Feb; 30 (2)– BIBEN RL, GORDAN GS.
Familial hypogonadotropic eunuchoidism. J Clin Endocrinol Metab. Aug; 15 (8)– Blackard WG, Heidingsfelder by: 6. The LH producing cell of the human adenohypophysis has been identified by the immunofluorescent technique followed by aldehyde thionin-periodic acid Schiff-orange G (AT-PAS-OG) staining.
Use of immunofluorescent stains demonstrates the clear differences in organization of FSH-ir cells (red) and LH-ir cells (green) at low magnification (B; ×). High magnification (×) confirms the virtually complete separation of LH and FSH immunoreactivity into separate populations of gonadotrophs (C), although cells that appeared to Cited by: Recent studies of human T cells in diverse tissue sites have revealed that the functional role of T cells is closely linked to the anatomical location, subset, and developmental stage.
Kumar et al. review these advances and highlight human-specific aspects of T cell by:. Stokes H, Boda JM () Immunofluorescent localization of growth hormone and prolactin in the adenohypophysis of fetal sheep. Endocrinology – Google Scholar Thompson SA, Trimble JJ () Immunohistochemical localization of prolactin cells of the pars distalis in the fetal and neonatal by: Since hormone producing cells of the adenohypophysis as well as ACTH- GH- and PRL-immunopositive adenomas express SHH and its target GLI1, Cited by: Here is an overview of immunofluorescence (IF) protocols.
Unfortunately, there isn't one protocol that is best for everything, so some testing and optimization is often necessary. If you can find out conditions that work well for your antibody-protein-specimen (eg from papers, companies selling the antibodies, lab web pages) that can save some time.